Sero-diagnosis of tuberculosis with A60 antigen enzyme-linked immunosorbent assay: failure in HIV-infected individuals in Ghana
Identifieur interne : 001475 ( Main/Exploration ); précédent : 001474; suivant : 001476Sero-diagnosis of tuberculosis with A60 antigen enzyme-linked immunosorbent assay: failure in HIV-infected individuals in Ghana
Auteurs : T. S. Van Der Werf [Ghana, Pays-Bas] ; P. K. Das [Pays-Bas] ; D. Van Soolingen [Pays-Bas] ; S. Yong [Pays-Bas] ; T. W. Van Der Mark [Pays-Bas] ; R. Van Den Akker [Pays-Bas]Source :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 1992-05-01.
Descripteurs français
- KwdFr :
- Adulte, Anticorps anti-VIH (sang), Anticorps antibactériens (sang), Antigènes bactériens, Erreurs de diagnostic, Humains, Immunoglobuline G (sang), Infections à VIH (), Infections à VIH (immunologie), Sensibilité et spécificité, Test ELISA (), Tuberculose pulmonaire (), Tuberculose pulmonaire (diagnostic), Tuberculose pulmonaire (immunologie), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie), VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie).
- MESH :
- Pascal (Inist)
- Wicri :
- geographic : Ghana.
- topic : Homme, Protection de l'environnement, Santé publique.
English descriptors
- KwdEn :
- Adult, Agogo, Agogo hospital, Anda biologicals, Antibodies, Bacterial (blood), Antigens, Bacterial, Assay, Cocito, Control subjects, Diagnosis, Diagnostic Errors, Diagnostic kits, Diagnostic usefulness, Dutch patients, ELISA assay, Elisa, Environmental protection, Enzyme immunoassay, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (statistics & numerical data), Forest area, Ghana, Ghanaian, Ghanaian controls, HIV Antibodies (blood), HIV Infections (complications), HIV Infections (immunology), HIV-1 (immunology), HIV-2 (immunology), Healthy controls, Higher levels, Human, Humans, Humoral, Immunoglobulin G (blood), Immunol, Immunosorbent, Immunosorbent assay, Kit, Median, Microbiol immunol, Mycobacterial, Mycobacterial antigen, Mycobacterium boris, National institute, Other mycobacterial diseases, Performance evaluation, Public health, Sensitivity and Specificity, Serological test, Serology, Serum, Serum antibody assay, Test results, Tuberculosis, Tuberculosis, Pulmonary (complications), Tuberculosis, Pulmonary (diagnosis), Tuberculosis, Pulmonary (immunology).
- MESH :
- chemical , blood : Antibodies, Bacterial, HIV Antibodies, Immunoglobulin G.
- complications : HIV Infections, Tuberculosis, Pulmonary.
- diagnosis : Tuberculosis, Pulmonary.
- immunology : HIV Infections, HIV-1, HIV-2, Tuberculosis, Pulmonary.
- methods : Enzyme-Linked Immunosorbent Assay.
- statistics & numerical data : Enzyme-Linked Immunosorbent Assay.
- Adult, Antigens, Bacterial, Diagnostic Errors, Humans, Sensitivity and Specificity.
- Teeft :
- Agogo, Agogo hospital, Anda biologicals, Assay, Cocito, Control subjects, Diagnostic kits, Diagnostic usefulness, Dutch patients, Elisa, Environmental protection, Enzyme immunoassay, Forest area, Ghana, Ghanaian, Ghanaian controls, Healthy controls, Higher levels, Humoral, Immunol, Immunosorbent, Immunosorbent assay, Median, Microbiol immunol, Mycobacterial, Mycobacterial antigen, Mycobacterium boris, National institute, Other mycobacterial diseases, Public health, Serological test, Serum antibody assay, Test results, Tuberculosis.
Abstract
Abstract: In order to assess the diagnostic usefulness of the A60 (ANDA Biologicals, Strassbourg, France) sero-diagnostic enzyme-linked immunosorbent assay (ELISA) kit for tuberculosis in Africa, sera of 53 pulmonary smear-positive tuberculosis (TB) patients, 30 apparently healthy control subjects and 6 AIDS suspects were sampled in Agogo Hospital in the forest area of Ghana. These sera were analyzed for antibodies to HIV-1 and HIV-2, and IgG-antibodies to the A60 BCG-antigen, while the non-HIV individuals were tested for total IgG levels. One healthy control subject, all of 6 AIDS suspects and 7 of the TB patients has HIV infections. In the non-HIV TB group, the sensitivity and specifity of the A60 ELISA was 78% and 86%, respectively, which was much poorer than expected from published reports about the A60 test. The A60 test failed, completely however, to discriminate between TB and non-TB in the HIV-positive group. In the non-HIV groups, total IgG levels were significantly higher in TB patients than in controls. It seems that the usefulness of the A60 ELISA test to diagnose tuberculosis is very limited in this high-incidence area, and that it seems to be of no value in patients infected with HIV.
Url:
DOI: 10.1007/BF00189425
Affiliations:
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Le document en format XML
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S." last="Van Der Werf">T. S. Van Der Werf</name><affiliation wicri:level="1"><country xml:lang="fr">Ghana</country><wicri:regionArea>Agogo Hospital</wicri:regionArea></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pulmonary Diseases, Groningen University Hospital, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P. K." last="Das">P. K. Das</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Departments of Dermatology and Pathology, Academic Medical Center, Meibergdreef 9, NL-1105 AZ, Amsterdam</wicri:regionArea><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Departments of Microbiology and Virology, National Institute of Public Health and Environmental Protection, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Van Soolingen, D" sort="Van Soolingen, D" uniqKey="Van Soolingen D" first="D." last="Van Soolingen">D. Van Soolingen</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Departments of Microbiology and Virology, National Institute of Public Health and Environmental Protection, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Yong, S" sort="Yong, S" uniqKey="Yong S" first="S." last="Yong">S. Yong</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Departments of Dermatology and Pathology, Academic Medical Center, Meibergdreef 9, NL-1105 AZ, Amsterdam</wicri:regionArea><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation></author><author><name sortKey="Van Der Mark, T W" sort="Van Der Mark, T W" uniqKey="Van Der Mark T" first="T. W." last="Van Der Mark">T. W. Van Der Mark</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pulmonary Diseases, Groningen University Hospital, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Van Den Akker, R" sort="Van Den Akker, R" uniqKey="Van Den Akker R" first="R." last="Van Den Akker">R. Van Den Akker</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Departments of Microbiology and Virology, National Institute of Public Health and Environmental Protection, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Medical Microbiology and Immunology</title><title level="j" type="abbrev">Med Microbiol Immunol</title><idno type="ISSN">0300-8584</idno><idno type="eISSN">1432-1831</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1992-05-01">1992-05-01</date><biblScope unit="volume">181</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="71">71</biblScope><biblScope unit="page" to="76">76</biblScope></imprint><idno type="ISSN">0300-8584</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8584</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Agogo</term><term>Agogo hospital</term><term>Anda biologicals</term><term>Antibodies, Bacterial (blood)</term><term>Antigens, Bacterial</term><term>Assay</term><term>Cocito</term><term>Control subjects</term><term>Diagnosis</term><term>Diagnostic Errors</term><term>Diagnostic kits</term><term>Diagnostic usefulness</term><term>Dutch patients</term><term>ELISA assay</term><term>Elisa</term><term>Environmental protection</term><term>Enzyme immunoassay</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (statistics & numerical data)</term><term>Forest area</term><term>Ghana</term><term>Ghanaian</term><term>Ghanaian controls</term><term>HIV Antibodies (blood)</term><term>HIV Infections (complications)</term><term>HIV Infections (immunology)</term><term>HIV-1 (immunology)</term><term>HIV-2 (immunology)</term><term>Healthy controls</term><term>Higher levels</term><term>Human</term><term>Humans</term><term>Humoral</term><term>Immunoglobulin G (blood)</term><term>Immunol</term><term>Immunosorbent</term><term>Immunosorbent assay</term><term>Kit</term><term>Median</term><term>Microbiol immunol</term><term>Mycobacterial</term><term>Mycobacterial antigen</term><term>Mycobacterium boris</term><term>National institute</term><term>Other mycobacterial diseases</term><term>Performance evaluation</term><term>Public health</term><term>Sensitivity and Specificity</term><term>Serological test</term><term>Serology</term><term>Serum</term><term>Serum antibody assay</term><term>Test results</term><term>Tuberculosis</term><term>Tuberculosis, Pulmonary (complications)</term><term>Tuberculosis, Pulmonary (diagnosis)</term><term>Tuberculosis, Pulmonary (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Anticorps anti-VIH (sang)</term><term>Anticorps antibactériens (sang)</term><term>Antigènes bactériens</term><term>Erreurs de diagnostic</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Infections à VIH ()</term><term>Infections à VIH (immunologie)</term><term>Sensibilité et spécificité</term><term>Test ELISA ()</term><term>Tuberculose pulmonaire ()</term><term>Tuberculose pulmonaire (diagnostic)</term><term>Tuberculose pulmonaire (immunologie)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Bacterial</term><term>HIV Antibodies</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="complications" xml:lang="en"><term>HIV Infections</term><term>Tuberculosis, Pulmonary</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Tuberculosis, Pulmonary</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Tuberculose pulmonaire</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Infections à VIH</term><term>Tuberculose pulmonaire</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>HIV Infections</term><term>HIV-1</term><term>HIV-2</term><term>Tuberculosis, Pulmonary</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps anti-VIH</term><term>Anticorps antibactériens</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Antigens, Bacterial</term><term>Diagnostic Errors</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Adulte</term><term>Antigènes bactériens</term><term>Diagnostic</term><term>Erreurs de diagnostic</term><term>Evaluation performance</term><term>Homme</term><term>Humains</term><term>Infections à VIH</term><term>Kit</term><term>Sensibilité et spécificité</term><term>Sérologie</term><term>Sérum</term><term>Technique ELISA</term><term>Test ELISA</term><term>Tuberculose</term><term>Tuberculose pulmonaire</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Agogo</term><term>Agogo hospital</term><term>Anda biologicals</term><term>Assay</term><term>Cocito</term><term>Control subjects</term><term>Diagnostic kits</term><term>Diagnostic usefulness</term><term>Dutch patients</term><term>Elisa</term><term>Environmental protection</term><term>Enzyme immunoassay</term><term>Forest area</term><term>Ghana</term><term>Ghanaian</term><term>Ghanaian controls</term><term>Healthy controls</term><term>Higher levels</term><term>Humoral</term><term>Immunol</term><term>Immunosorbent</term><term>Immunosorbent assay</term><term>Median</term><term>Microbiol immunol</term><term>Mycobacterial</term><term>Mycobacterial antigen</term><term>Mycobacterium boris</term><term>National institute</term><term>Other mycobacterial diseases</term><term>Public health</term><term>Serological test</term><term>Serum antibody assay</term><term>Test results</term><term>Tuberculosis</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Protection de l'environnement</term><term>Santé publique</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In order to assess the diagnostic usefulness of the A60 (ANDA Biologicals, Strassbourg, France) sero-diagnostic enzyme-linked immunosorbent assay (ELISA) kit for tuberculosis in Africa, sera of 53 pulmonary smear-positive tuberculosis (TB) patients, 30 apparently healthy control subjects and 6 AIDS suspects were sampled in Agogo Hospital in the forest area of Ghana. These sera were analyzed for antibodies to HIV-1 and HIV-2, and IgG-antibodies to the A60 BCG-antigen, while the non-HIV individuals were tested for total IgG levels. One healthy control subject, all of 6 AIDS suspects and 7 of the TB patients has HIV infections. In the non-HIV TB group, the sensitivity and specifity of the A60 ELISA was 78% and 86%, respectively, which was much poorer than expected from published reports about the A60 test. The A60 test failed, completely however, to discriminate between TB and non-TB in the HIV-positive group. In the non-HIV groups, total IgG levels were significantly higher in TB patients than in controls. It seems that the usefulness of the A60 ELISA test to diagnose tuberculosis is very limited in this high-incidence area, and that it seems to be of no value in patients infected with HIV.</div></front></TEI><affiliations><list><country><li>Ghana</li><li>Pays-Bas</li></country><region><li>Groningue (province)</li><li>Hollande-Septentrionale</li></region><settlement><li>Amsterdam</li><li>Groningue</li></settlement></list><tree><country name="Ghana"><noRegion><name sortKey="Van Der Werf, T S" sort="Van Der Werf, T S" uniqKey="Van Der Werf T" first="T. S." last="Van Der Werf">T. S. Van Der Werf</name></noRegion></country><country name="Pays-Bas"><region name="Groningue (province)"><name sortKey="Van Der Werf, T S" sort="Van Der Werf, T S" uniqKey="Van Der Werf T" first="T. S." last="Van Der Werf">T. S. Van Der Werf</name></region><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P. K." last="Das">P. K. Das</name><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P. K." last="Das">P. K. Das</name><name sortKey="Van Den Akker, R" sort="Van Den Akker, R" uniqKey="Van Den Akker R" first="R." last="Van Den Akker">R. Van Den Akker</name><name sortKey="Van Der Mark, T W" sort="Van Der Mark, T W" uniqKey="Van Der Mark T" first="T. W." last="Van Der Mark">T. W. Van Der Mark</name><name sortKey="Van Soolingen, D" sort="Van Soolingen, D" uniqKey="Van Soolingen D" first="D." last="Van Soolingen">D. Van Soolingen</name><name sortKey="Yong, S" sort="Yong, S" uniqKey="Yong S" first="S." last="Yong">S. Yong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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